--Development of resistance to commonly used insecticides is a serious threat to our ability to combat mosquito-borne diseases such as dengue and malaria. Insecticide resistance surveillance is required to monitor its development and to take action in advance. As new compounds are in the market, appropriate methods to monitor insecticide resistance are necessary for those new molecules. The Wold Health Organization (WHO) is developing a multi-centre evaluation to determine and update insecticide discriminating concentrations for hose new insecticides, against different species of Anopheles and Aedes mosquitoes. In Colombia, Anopheles albimanus will be tested. For the new compounds, bottle assays will be developed, and also, WHO paper assays will be carried out, with different insecticides, in order to standardize their discriminating concentrations. This information is key to establish the baseline susceptibility of vector populations to these new insecticides and to detect any change in phenotypic resistance after their deployment.