Background: There is scarce information on candidemia in hospitalized patients in Latin America, outside of Brazil. Methods: Patients from seven tertiary care hospitals in Bogotá, Colombia, with isolation of Candida spp. from a blood culture were followed prospectively from March 2008 to March 2009. Epidemiogical information, risk factors, and mortality were prospectively collected. Isolates were sent to a reference center and fluconazole susceptibility was tested by agar based E-test. A comparison between results of susceptibility by using 2008 and 2012 break points was done. A multivariate analysis was used to determinate risk factors for mortality. Results: We identified 131 patients, with a median age of 41.2 years. Isolates were more frequently found in the intensive care unit (ICU). Candida albicans was the most prevalent species with 66.4% of the isolates, followed by C. parapsillosis (14%). Fluconazole resistance was found in 3.2% and 17.6% of the isolates with 2 different cutoffs (2008 and 2012 break points, respectively). Fluconazole was used as empirical antifungal therapy in 68.8% of the cases, followed by amphotericin B (22%). Hospital crude mortality was 35.9%. Mortality was associated with age and presence of shock at the time of Candida detection. Fluconazol therapy was recognized as a protector factor for mortality. Conclusions: Candidemia has a high mortality rate. Age and shock increase mortality while fluconazole use serve as a protector factor. A higher resistance rates with new break points was noted.