Soil genesis and functions are conditioned by the composition of mineral and organic materials whose interaction determines the efficiency of physical, chemical, and biological processes. Soil organic matter is an essential component of the soil and directly influences the soil health and quality. To determine the dynamics of organic matter in a Pachic Hydric Melanudand (Soil Survey Staff, 2022) under grassland (Pennisetum cladestinum), potato (Solanum tuberosum), and Andean paramo (Espeletia sp); its physical, chemical, and biological properties were evaluated. In this study, Bulk Density (BD), Total Porosity (TP), Aggregate Stability (AS), Soil Organic Matter (SOM), pH, Available Phosphorus (AP), Total Nitrogen (TN), Cation Exchange Capacity (CEC), Microbial Biomass (MB) and basal respiration (BR) were measured at three sampling depths (0-5 cm, 5-15 cm, and 15-30 cm) with three repetitions by land use. The results shown significant influence (p¿0.01) of non-organic amendments and fertilizers on the content of AP, TN, CEC, TP, MB, and BR. The SOM was linked to the availability of nutrients (p<0.01) as well as to the functioning of the pore space (TP p<0.01), with a direct impact of land use on the dynamics of the SOM. These results allow planning the anthropic activities that will be maintained in paramo ecosystems, revaluing those that modify the physical properties of the soil (degrading). In paramo ecosystems, it is possible to implement management or remediation processes in the future to conserve the provision of ecosystem services associated with the soil quality and land degradation.