Proyectos
Sliding motility, biofilm formation and glycopeptidolipid production of Mycobacterium colombiense strains
Resumen
Mycobacterium colombiense is a novel member of the Mycobacterium avium complex that produces respiratory and disseminated infections in immunosuppressed patients. At present, the morphologic and genetic bases involved in the phenotypic features of M. colombiense strains are completely unknown. In this study, we demonstrate that M. colombiense strains displaying smooth morphology have an increased ability both to form biofilms on a hydrophobic surface and to slide over motility plates. Thin-layer chromatography experiments showed that strains of M. colombiense displaying smooth colonies produce large amounts of glycolipids with a chromatographic behavior similar to that of glycopeptidolipids (GPLs) of M. avium. Conversely, we found a natural rough variant of M. colombiense (57B strain) that lacked pigmentation and showed an impaired capacity to slide, form biofilms and produce GPLs. Based on bioinformatic analyses, a gene cluster that is possibly involved in the GPL biosynthesis in M. colombiense CECT 3035 is proposed. RT-qPCR experiments showed that motile conditions of culture activate the transcription of some genes that are predicted to be involved in key enzymatic activities of the GPL biosynthesis.
Convocatoria
Nombre de la convocatoria:CONVOCATORIA DEL PROGRAMA NACIONAL PARA LA VISIBILIDAD INTERNACIONAL DE LA PRODUCCIÓN ACADÉMICA MEDIANTE EL APOYO PARA TRADUCCIÓN O CORRECCIÓN DE ESTILO DE ARTÍCULOS DE INVESTIGACIÓN 2013-2015
Modalidad:Modalidad 1. Revisión de estilo o traducción de artículos completos escritos por docentes de planta de la Universidad Nacional de Colombia, los cuales vayan a ser sometidos a revistas indexadas en ISI Web Of Knowledge o Scopus del Elsevier.
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