Proyectos
Sliding motility, biofilm formation and glycopeptidolipid production of Mycobacterium colombiense strains
Resumen
Mycobacterium colombiense is a novel member of the Mycobacterium avium complex that produces respiratory and disseminated infections in immunosuppressed patients. At present, the morphologic and genetic basis involved in the phenotypic features of M. colombiense strains are completely unknown. In this study, we demonstrate that M. colombiense strains displaying smooth morphology have an increased ability of both: biofilm formation on a hydrophobic surface and ability to slide over of motility plates. Thin-layer chromatography experiments showed that strains of M. colombiense displaying smooth colonies produce large amounts of glycolipids with a chromatographic behavior similar to glycopeptidolipids (GPLs) of M. avium. By contrast, a natural rough variant of M. colombiense (57B strain) exhibited deficiency to produce some pigmentation with age, impaired capacity to slide, to form biofilm and to produce GPLs. According to bioinformatic analyses, a gene cluster possibly involved in the GPLs biosynthesis in M. colombiense CECT 3035 is proposed; Real-time PCR and TLC experiments showed that motile conditions of culture activate the transcription of some genes predicted to be involved in the biosynthesis of M. colombiense GPLs, which correlates with the accumulation of this kind of glycolipids in the cell envelope.
Convocatoria
Nombre de la convocatoria:CONVOCATORIA DEL PROGRAMA NACIONAL PARA LA VISIBILIDAD INTERNACIONAL DE LA PRODUCCIÓN ACADÉMICA MEDIANTE EL APOYO PARA TRADUCCIÓN O CORRECCIÓN DE ESTILO DE ARTÍCULOS DE INVESTIGACIÓN 2013-2015
Modalidad:Modalidad 1. Revisión de estilo o traducción de artículos completos escritos por docentes de planta de la Universidad Nacional de Colombia, los cuales vayan a ser sometidos a revistas indexadas en ISI Web Of Knowledge o Scopus del Elsevier.
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