Pantoea ananatis, in the last decade appears closely associated with the microbiome and different bioactivities in hematophagous vector insects. In this study, the genome sequence of a Pantoea ananatis strain 140-UN obtained from the digestive tract of Pintomyia evansi (hematophagous insect) was accessed through a comparative approach on genomic and taxonomic variability and the relationship of gene regions with previously characterized In vitro bioactivities. The resulting scaffold-level genome of P. ananatis has a total length of 4¿929.135 bp, of which 4690 attributes were annotated. The genome of P. ananatis 140-UN is mainly made up of a chromosome and a plasmid (LPP-1) with a size of 308.225 bp with a G + C content of 53.2%. The P. ananatis strain 140-UN is closely related to P. ananatis strain SGAir0210 obtained from an air collector. Pan-genome analysis shows regions that are not represented in other genomes previously described. Genomic analysis of antimicrobial resistance factors showed genes that conferred resistance to aminoglycosides. Unique regions were also identified in the strain corresponding to 888 genes (373 unique genes) mainly related to genetic information processing and signaling and cellular processes. Such variability may be due, in part, to the possible presence of various inserted phage regions. These unique genes, together with the previously reported genes found of biotechnological interest (siderophores, thiopeptides, homoserine lactones, and terpenes) are key to understanding activities such as cell signaling, enzymatic and antimicrobial activities, resistance against antibiotics, and possible activities against pathogens, as well as the specific organism-host relationship that occurs with the vector insect within the gastrointestinal compartment.