Proyectos
The DosR dormancy regulator of Mycobacterium tuberculosis stimulates the Na+/K+ and Ca2+ ATPase activities in plasma membrane vesicles from mycobacteria
Resumen
The latency global regulator DosR regulon of M. tuberculosis that is stimulated by hypoxia comprises approximately fifty genes including those that codify for putative plasma membrane P-type ATPases. In this work, the influence of hypoxia and the M. tuberculosis DosR on the ATPase activity of M. smegmatis mc2155 plasma membrane was assessed. We found that the basal ATPase activity of plasma membrane vesicles from cells cultured under hypoxia decreased 30% approximately in comparison to the displayed by oxygenated cells. By contrast, the specific Na+/K+ and Ca2+ ATPase activities of plasma membrane increased 2.8 and 3.5-fold respectively, under hypoxia similar to the observed for cells over-expressing the DosR regulator. In addition, we performed bioinformatics analysis that indicated that the Pma1 gene product is a possible alkaline/alkaline earth metal cation P-type ATPase transporter of M. smegmatis. In agreement, RT-qPCR experiments demonstrated that the transcription level of pma1 gene increased under hypoxia at levels similar to M. smegmatis cells over-expressing the M. tuberculosis DosR regulator. The whole findings suggest that hypoxia induces the Na+/K+ and Ca2+ ATPase activity in mycobacterial plasma membrane possibly mediated by the dormancy regulator DosR.
Convocatoria
Nombre de la convocatoria:CONVOCATORIA DEL PROGRAMA NACIONAL PARA LA VISIBILIDAD INTERNACIONAL DE LA PRODUCCIÓN ACADÉMICA MEDIANTE EL APOYO PARA TRADUCCIÓN O CORRECCIÓN DE ESTILO DE ARTÍCULOS DE INVESTIGACIÓN 2013-2015
Modalidad:Modalidad 1. Revisión de estilo o traducción de artículos completos escritos por docentes de planta de la Universidad Nacional de Colombia, los cuales vayan a ser sometidos a revistas indexadas en ISI Web Of Knowledge o Scopus del Elsevier.
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