Syn-R-Gene - Synthetic gene-regulatory networks in microorganisms
The analysis of regulatory networks that control gene ex pression in biological systems is one of the challenges in current research. Besides indifying the entities of such networks the understanding of their dynamics is in most cases a still insuperable obstacle. Artificial gene-regulatory networks in microorganisms may provide a way out to gain experiences of their dynamical behavior. Networks - starting from very simple ones and increasing in their complexity in the following steps - can be engineered artificially in organisms that are easily manipulated (e.g. bacterias or yeasts). The behavior of such artificial networks is monitored by reporter genes, e.g. the green flurescent protein GFP or its derivates YFP and CFP. In the framework of the project proposed here we plan to generate artificial gene-regulatory networks in bacteria and yeast cells. These networks will integrate external stimuli and produce stimulidependent outputs. The dynamic behavior of the networks will be analyzed and the results will be compared with those of computational simulations. Specifically, we would like to address two main questions: (i) Is it possible to calculate with eukaryotic microorganisms? (ii) Is it possible to synthetically synchronize a population of oscillators that were artificially introduced into Escherichia coli? The Colombian and German groups optimally complement each other in their background and their methodical approaches. The involved partners have complementary backgrounds from microbiology, physics, mathematics and molecular biology. The proposed projects were initiated and developed during a short-term stay of the Colombian partner in Potsdam in autumn 2007 and were improved during the visit of some of the German partners in Bogotá at the beginning of 2008.
Nombre de la convocatoria:Proyectos Jornada Docente
Modalidad:Proyectos Jornada Docente