Plasmodium falciparum is one parasite responsible of malaria, a disease causing of thousands of deaths every year. Treatment of malaria is very difficult because of parasites generate resistance to conventional drugs. Nowadays, researchers are focused on developing new treatments to control it. A possible alternative are the secondary metabolites produced by neem tree (Azadirachta indica) which have shown in vitro antiplasmodial activity. The in vitro antiplasmodial activity of extract and some fractions from A. indica cell culture was evaluated on chloroquine-resistant P. falciparum (FCR3) strains. The activity showed by the extract was promising (IC50 6.14 ¿g/ml). IC50 of hexane fraction was 13.66 ¿g/ml and dichloromethane fraction, 9.80 ¿g/ml. As far as our knowledge, this is the first report that shows the antiplasmodial effect of extract from A. indica cell culture. With these results, this extract could be profiled as future alternative to treat and control malaria.